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Transgenic Services
HDB Functional Genetic Center (HDB-FTC) is a custom request-based platform providing full-scale service from gene cloning, making gene expression vector/targeting construct, pronuclear/blastocyst microinjection, genotyping, to characterizations of phenotypes at both in vitro and in vivo levels in the mouse, among which the last capacity/feasibility
differentiates HDB-FTC from other transgenic resources.
Contents of service



Fig. 1. Generation of knockout mice by homologous recombination. A. Chimeric mice between 129/SEV ES cells (agouti) and C57BL6 (J6) blastocysts (black). B. Potential germline transmission of their pups with agouti fur color.
Making expression vector for producing transgenic mice, making gene targeting construct for producing knockout, knockdown, and knock-in mice, and constructing BAC clones containing haplotype(s) or vulnerable loci for a particular type of disease for generating
BAC transgenic mice
Homologous recombination, ES cell screening, determining transgene copy number in transgenic founder mice
Mouse breeding, mouse genotyping, and determining heterozygous/homozygous in
knockout/down/in mice, and hemizygous/homozygous in transgenic mice
Basic characterization of mutant mice at molecular and histological levels
Phenotypical characterization of mutant mice in various systems including behaviors, cancer biology and oncogene, cardiovascular system, CNS, immunology, inflammation and infection, metabolic
disease, and other systems
Types of genetic manipulations
Common (conventional) knockout, knockdown, and knock-in mice
Fig. 2. BDNF knockdown mice. An obese
phenotype is observed in knockdown mice (left), compared to wild-type mice (right).
Common transgenic mice
Conditional, brain region-specific, organ-specific, and cell-type-specific knockout mice
Brain region-specific and cell type-specific inducible/reversible transgenic mice
BAC/YAC transgenic mice
Small RNA manipulation
Zinc finger nuclease in rat and cell lines (under development)
Fig. 3. Inducible/reversible forebrain-specific
transgenic mice. A.
wild-type mice-treated with vehicle.
B. transgenic mice-treated with vehicle. C. Transgenic
mice mice-treated with doxy for 3 days. D. Transgenic
mice-withdrawn doxy for 5 days after 3-day-doxy
treatment.
Fig. 4. Cell type-specific expression of a
reporter gene (Lac-Z). A.
Cerebellum-specific. B.
Cerebellar cortex-specific. C. Cerebellar Purkinjes
cell-specific. D. Cerebellar granule cell-specific
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
[email protected]
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